Streptococcus agalactiae Strains with Chromosomal Deletions Evade Detection with Molecular Methods

Author(s)

Isabella A. Tickler, Cepheid
Fred C. Tenover, CepheidFollow
Scott Dewell, Cepheid
Victoria M. Le, Cepheid
Rachel N. Blackman, Cepheid
Richard V. Goering, Creighton University
Amy E. Rogers, Women's Hospital, Baton Rouge
Heather Piwonka, Willis-Knighton Health System
Brittney D. Jung-Hynes, University of Wisconsin - Madison
Derrick J. Chen, University of Wisconsin - Madison
Michael J. Loeffelholz, Cepheid
Devasena Gnanashanmugam, Cepheid
Ellen Jo Baron, Cepheid

Document Type

Article

Publication Date

4-2019

Publication Source

Journal of Clinical Microbiology

Abstract

Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of Streptococcus agalactiae (group B Streptococcus [GBS]) from several geographic locations in the United States and Ireland that contain deletions in or adjacent to the region of the chromosome that encodes the hemolysin gene cfb, the region targeted by the Xpert GBS and GBS LB assays. PCR-negative, culture-positive isolates were recognized during verification studies of the Xpert GBS assay in 12 laboratories between 2012 and 2018. Whole-genome sequencing of 15 GBS isolates from 11 laboratories revealed four unique deletions of chromosomal DNA ranging from 181 bp to 49 kb. Prospective surveillance studies demonstrated that the prevalence of GBS isolates containing deletions in the convenience sample was

ISBN/ISSN

Print: 0095-1137; Electronic: 1098-660X

Document Version

Postprint

Comments

The document available for download is the authors' accepted manuscript, provided to PubMed Central with the permission of the publisher. Permission documentation is on file.

PMCID: PMC6440789

PMID: 30760532

To view the version of record, use the DOI: https://doi.org/10.1128/jcm.02040-18

Publisher

American Society for Microbiology

Volume

57

Peer Reviewed

yes

Issue

4

Keywords

Streptococcus agalactiae, group B streptococcus, whole-genome sequencing

eCommons Citation

Tickler, Isabella A.; Tenover, Fred C.; Dewell, Scott; Le, Victoria M.; Blackman, Rachel N.; Goering, Richard V.; Rogers, Amy E.; Piwonka, Heather; Jung-Hynes, Brittney D.; Chen, Derrick J.; Loeffelholz, Michael J.; Gnanashanmugam, Devasena; and Baron, Ellen Jo, "Streptococcus agalactiae Strains with Chromosomal Deletions Evade Detection with Molecular Methods" (2019). Biology Faculty Publications. 343.
https://ecommons.udayton.edu/bio_fac_pub/343