Title

A Spectroscopic, Photocalorimetric, and Theoretical Investigation of the Quantum Efficiency of the Primary Event in Bacteriorhodopsin

Document Type

Article

Publication Date

5-1989

Publication Source

Journal of the American Chemical Society

Abstract

The spectroscopic, photochemical, and energetic properties of the primary event of light-adapted bacteriorhodopsin (bR) are investigated with pulsed laser cryogenic photocalorimetry, photostationary-state spectral analysis, INDO-PSDCI molecular orbital theory, and semiempirical molecular dynamics theory. The principal goal is to explore the photophysical origins of the controversy concerning the primary quantum yield. ... Experimental and theoretical evidence is presented which suggests that two distinct forms of light-adapted bacteriorhodopsin may exist. We propose that these two forms have characteristic photocycles with significantly different primary quantum yields.

Inclusive pages

4063-4074

ISBN/ISSN

0002-7863

Comments

Permission documentation on file.

Publisher

American Chemical Society

Volume

111

Peer Reviewed

yes

Issue

11

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