Document Type
Article
Publication Date
11-2014
Publication Source
Colloids and Surfaces B: Biointerfaces
Abstract
Due to their distinctive physiochemical properties, including a robust antibacterial activity and plasmonic capability, hundreds of consumer and medical products contain colloidal silver nanoparticles (AgNPs). However, even at sub-toxic dosages, AgNPs are able to disrupt cell functionality, through a yet unknown mechanism. Moreover, internalized AgNPs have the potential to prolong this disruption, even after the removal of excess particles. In the present study, we evaluated the impact, mechanism of action, and continual effects of 50 nm AgNP exposure on epidermal growth factor (EGF) signal transduction within a human keratinocyte (HaCaT) cell line. After AgNP expose, EGF signaling was initially obstructed due to the dissolution of particles into silver ions. However, at longer durations, the internalized AgNPs increased EGF signaling activity. This latter behavior correlated to sustained HaCaT stress, believed to be maintained through the continual dissolution of internalized AgNPs. This study raises concerns that even after exposure ceases, the retained nanomaterials are capable of acting as a slow-release mechanism for metallic ions; continually stressing and modifying normal cellular functionality.
Inclusive pages
136–142
ISBN/ISSN
0927-7765
Document Version
Postprint
Copyright
Copyright © 2014, Elsevier
Publisher
Elsevier
Volume
123
Peer Reviewed
yes
Issue
1
Sponsoring Agency
University of Dayton; Henry M. Jackson Foundation
eCommons Citation
Comfort, Kristen K.; Maurer, Elizabeth I.; and Hussain, Saber M., "Slow Release of Ions from Internalized Silver Nanoparticles Modifies the Epidermal Growth Factor Signaling Response" (2014). Chemical and Materials Engineering Faculty Publications. 174.
https://ecommons.udayton.edu/cme_fac_pub/174
Included in
Other Chemical Engineering Commons, Other Materials Science and Engineering Commons, Petroleum Engineering Commons, Polymer and Organic Materials Commons, Thermodynamics Commons
Comments
The document available for download is the authors' accepted manuscript, provided in compliance with the publisher's policy on self-archiving. Some differences may be present between the document and the version of record.
Permission documentation is on file.