Title

Apoptosis: A Mechanism Contributing to Remodeling of Skeletal Muscle in Response to Hindlimb Unweighting

Document Type

Article

Publication Date

8-1-1997

Publication Source

American Journal of Physiology - Cell Physiology

Abstract

The role of apoptosis in the elimination of myonuclei during hindlimb unloading-induced atrophy and the inhibition of apoptosis in the prevention of muscle atrophy were examined. The number of nuclei demonstrating double-stranded DNA fragmentation seen by terminal deoxynucleotidyl transferase (TDT) histochemical staining, an indicator of apoptosis, was significantly increased after 14 days of suspension. Double staining with TDT and antilaminin immunohistochemistry revealed that some TDT-positive nuclei were within the fiber lamina and were most likely myonuclei. The number of fibers containing morphologically abnormal nuclei was also significantly greater in suspended compared with control rats. Combined treatment with growth hormone and insulin-like growth factor I (GH/ IGF-I) and resistance exercise attenuated the increase in TDT-positive nuclei (approximately 26%, P > 0.05) and significantly decreased the number of fibers with morphologically abnormal nuclei. The data suggest that 1) “programmed nuclear death” contributes to the elimination of myonuclei and/or satellite cells from atrophying fibers, and 2) GH/IGF-I administration plus muscle loading ameliorates the apoptosis associated with hindlimb unloading.

Inclusive pages

C579-C587

ISBN/ISSN

0363-6143

Comments

Permission documentation on file.

Publisher

American Physiological Society

Volume

273

Issue

2

Peer Reviewed

yes

Link to Full Text

Share

COinS