This is a data archive of 41 years of permanent plot data from Hueston Woods State Nature Preserve, an old-growth beech-maple forest in Preble County, Ohio. The long-term monitoring of forests using repeated sampling in permanent plots is an effective way to assess vegetation dynamics and community disturbance responses. The project was founded in 1979 by Dr. John Vankat, who taught and researched plant ecology at Miami University for 31 years. The plot was sampled four times, in 1981, 1988, 1994, and 2000, before being passed on to Dr. Ryan McEwan at the University of Dayton. The plot was last sampled in 2022. The data can be used to explore geospatial and demographic patterns of woody vegetation change.