Using novel Fly-FUCCI system to evaluate tumor growth and progression

Using novel Fly-FUCCI system to evaluate tumor growth and progression

Presenter(s)

Kirti Snigdha, Mitchell L Stanley

Description

The FUCCI system (fluorescent ubiquitination-based cell cycle indicator) has been proven to be a promising approach for in vivo studies of cell proliferation. Using Drosophila-specific FUCCI system (Fly-FUCCI) allows one to distinguish between G1, G2, and S phases. Fly-FUCCI relies on fluorochrome-tagged degrons, which are degraded during mitosis or the onset of S phase, respectively. Fly-FUCCI allows one to track cell-cycle patterns in cultured Drosophila cells, eye and wing imaginal discs, salivary glands, the adult midgut, and other tissues. The FUCCI system can be utilized with Drosophilacancerous tissues to identify when tumor growth is most prominent or suppressed during cell cycles. This information can be used to understand the starting stages of cancer within cells, what stages of cell proliferation are necessary for tumor growth, and how the nutrient growth of normal cells compares to the grow cancerous cells. The Fly-FUCCI system is an invaluable tool for visualizing cell-cycle activity duringdevelopment, tissue homeostasis, and abnormal cell development. In the lab, Drosophila melanogaster is used to understand the intricacies of cancer biology using genetically induced tumors in the different imaginal discs and brain. Using Fly-FUCCI system, allows one to evaluate the cell cycle differences during the tumor growth and progression. Here are the present finding on utilization of this technique to evaluate tumor cell cycle regulation

Publication Date

4-5-2017

Project Designation

Independent Research - Graduate

Primary Advisor

Madhuri Kango-Singh, Amit Singh

Primary Advisor's Department

Biology

Keywords

Stander Symposium project

Using novel Fly-FUCCI system to evaluate tumor growth and progression

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