Inhibition of Pseudomonas aeruginosa Biofilm Growth using TMP preventative treatment on Steel and Polyethylene substrata

Inhibition of Pseudomonas aeruginosa Biofilm Growth using TMP preventative treatment on Steel and Polyethylene substrata

Presenter(s)

Erin Elise Pellot

Description

Within recent years there have been a growing number of antibiotic resistant bacterial strains. Many of these strains produce biofilms, which are sturdy layers of bacteria on a surface. These biofilms are highly antibiotic resistant and have multiple defense mechanisms. Biofilms have been known to form layers on metal implants after surgery and dialysis tubing which can prevent typical antibiotic treatment methods from being effective. In order to prevent biofilms from forming on steel and polyethylene coupons the porphyrin Tetramesitylporphyrin (TMP) was tested as a pre-treatment for Pseudomonas aeruginosa, one of the six ESKAPE bacterial pathogens responsible for most deadly opportunistic infections in the United States. In this experiment steel and polyethylene coupons were placed in a 225 µM TMP and a 100 µM TMP solution for time periods of three and five hours. After this presoak period the coupons were submerged in a PAO1 liquid culture for an 18-hour period. Once a biofilm was formed the coupons were removed and vortexed in sterile water to resuspend the biofilm. A serial dilution and plating was then carried out and the plates were placed in the 37°C incubator overnight. A Colony forming unit (CFU) count was taken the next day to asses the log reduction created by TMP pretreatment. Results showed a roughly .5 log reduction in the 100 µM TMP and 225 µM TMP treatment trials.

Publication Date

4-24-2019

Project Designation

Independent Research

Primary Advisor

Karolyn M. Hansen, Nehaben Nik Patel

Primary Advisor's Department

Biology

Keywords

Stander Symposium project

Inhibition of Pseudomonas aeruginosa Biofilm Growth using TMP preventative treatment on Steel and Polyethylene substrata

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