Establishing an Immunohistochemical Method to Assess Cell Activation in the Amphibian Nervous System
Presenter(s)
Ben Klocke, Kaitlyn Martin, Augustine J. Miller, Jason Tornes
Files
Description
According to the National Institute of Health, 185,000 people undergo amputations each year in the United States. Understanding the process of regeneration is imperative in order to develop novel therapies and treatments for these patients. Unlike most vertebrates, axolotls can fully regenerate their limbs when amputated. In the context of the current study, we established and tested a new immunohistochemical protocol in our lab by which we can track the activation of cell cycle in different tissues in the axolotl during regeneration upon injection of 5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue which is incorporated into the DNA of dividing cells. Specifically, we utilized double immunofluorescence and confocal microscopy in paraffin sections to assess cell proliferation in specific cell types of interest. A new protocol was successfully established in our lab, and we are continuing data collection to study the implication of the nervous system in this remarkable process.
Publication Date
4-20-2022
Project Designation
Course Project
Primary Advisor
Pothitos Pitychoutis
Primary Advisor's Department
Biology
Keywords
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being
Recommended Citation
"Establishing an Immunohistochemical Method to Assess Cell Activation in the Amphibian Nervous System" (2022). Stander Symposium Projects. 2410.
https://ecommons.udayton.edu/stander_posters/2410
Comments
Presentation: 11:20 a.m.-11:40 a.m., Kennedy Union 311
This project reflects research conducted as part of a course project designed to give students experience in the research process.
Course: BIO 421
External collaborator:
Dr. Katia Del Rio-Tsonis, Miami University (Oxford, OH)