Establishing an Immunohistochemical Method to Assess Cell Activation in the Amphibian Nervous System
Ben Klocke, Kaitlyn Martin, Augustine J. Miller, Jason Tornes
According to the National Institute of Health, 185,000 people undergo amputations each year in the United States. Understanding the process of regeneration is imperative in order to develop novel therapies and treatments for these patients. Unlike most vertebrates, axolotls can fully regenerate their limbs when amputated. In the context of the current study, we established and tested a new immunohistochemical protocol in our lab by which we can track the activation of cell cycle in different tissues in the axolotl during regeneration upon injection of 5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue which is incorporated into the DNA of dividing cells. Specifically, we utilized double immunofluorescence and confocal microscopy in paraffin sections to assess cell proliferation in specific cell types of interest. A new protocol was successfully established in our lab, and we are continuing data collection to study the implication of the nervous system in this remarkable process.
Primary Advisor's Department
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being
"Establishing an Immunohistochemical Method to Assess Cell Activation in the Amphibian Nervous System" (2022). Stander Symposium Projects. 2410.