i-TRACE RNA interference-based reporter system to distinguish spatiotemporal gene expression in real time versus lineage cells in Drosophila eye

i-TRACE RNA interference-based reporter system to distinguish spatiotemporal gene expression in real time versus lineage cells in Drosophila eye

Presenter(s)

Anuradha Chimata Venkatakrishnan, Paige Elizabeth McBride

Comments

Presentation: 9:00 a.m.-10:15 a.m., Kennedy Union Ballroom

This project reflects research conducted as part of a course project designed to give students experience in the research process.

Course: BIO 300

Description

Dynamic gene expression pattern along the spatiotemporal axis is a key fundamental feature of all cells. Such changes contribute to cellular responses to stimuli and also execution of highly regulated and sequential developmental programs. During Drosophila (a.k.a fruit fly) eye development, several genes are expressed in a dynamic manner to regulate target genes. Our interest was to understand the exact expression pattern of some of the genes involved in this process. We have used the i-TRACE (RNAi Technique for Real-time And Clonal Expression) system to assess such minute but dynamic changes in expression pattern by combining Gal4/UAS, FLP/FRT, RNAi and fluorescent reporters. Upon crossing with a Gal4 stock, all real time gene expression (Gal4 mediated) is marked by the presence of red fluorescent protein (RFP) and all lineage cells express green fluorescent protein (GFP) independent of Gal4 activity. Additionally, the effect of Gal4 mediated GFP-RNAi expression persists in cells that currently and recently expressed the gene. This enables us to observe even brief changes in expression marked by loss of GFP. Here, we present the expression data of some of the genes that play an important role during Drosophila eye development.

Publication Date

4-20-2022

Project Designation

Course Project

Primary Advisor

Amit Singh

Primary Advisor's Department

Biology

Keywords

Stander Symposium project, College of Arts and Sciences

United Nations Sustainable Development Goals

Good Health and Well-Being

i-TRACE RNA interference-based reporter system to distinguish spatiotemporal gene expression in real time versus lineage cells in Drosophila eye

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