Using a Drosophila Glioma Model to Study Signaling Pathways

Using a Drosophila Glioma Model to Study Signaling Pathways

    Presenter(s)

    Jibriel Saqibuddin

    Comments

    Presentation: 1:15 p.m.-2:30 p.m., Kennedy Union Ballroom

    This project reflects research conducted as part of a course project designed to give students experience in the research process.

    Course: BIO 421

    Additional authors:
    Jibriel Saqibuddin1, Arushi Rai1, B. N. Rohith1, Amit Singh1,2, Madhuri Kango-Singh1,2

    1: Department of Biology, University of Dayton, Dayton, OH
    2: Integrative Science and Engineering Center, Dayton, OH

    Description

    Drosophila melanogaster is an ideal genetic model for studying early changes in tumor cells for diseases such as Glioblastoma (GBM) for which there are no current treatment options. A large percent of GBM patients show excess EGFR signaling due to amplification of Epidermal Growth Factor Receptor-VIII (EGFR-VIII), and activation of the phosphatidylinositol 3-kinase (PI3K) pathway. Using mutations to activate the PI3K and EGFR receptors in Drosophila, we have modeled GBM in the fly brain using the GAL4-UAS system.My project involves genetic crosses that will produce larvae with GBM, the dissections of the larval brains, and performing immunohistochemistry to study changes in signaling pathways that promote GBM growth. To do so, I will be studying the early time points to decipher the roles of signaling pathways like MAPK, Hippo and WNT in promoting GBM growth and/or inhibiting cell death. A comparison between our GBM models and experimental controls will provide the initial data for designing further genetic experiments to narrow down specific signaling interactions that affect cell death and proliferation.

    Publication Date

    4-20-2022

    Project Designation

    Course Project

    Primary Advisor

    Madhuri Kango-Singh

    Primary Advisor's Department

    Biology

    Keywords

    Stander Symposium project, College of Arts and Sciences

    United Nations Sustainable Development Goals

    Good Health and Well-Being

    Using a Drosophila Glioma Model to Study Signaling Pathways

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