Using a Drosophila Glioma Model to Study Signaling Pathways

Using a Drosophila Glioma Model to Study Signaling Pathways

Presenter(s)

Jibriel Saqibuddin

Comments

Presentation: 1:15 p.m.-2:30 p.m., Kennedy Union Ballroom

This project reflects research conducted as part of a course project designed to give students experience in the research process.

Course: BIO 421

Additional authors:
Jibriel Saqibuddin1, Arushi Rai1, B. N. Rohith1, Amit Singh1,2, Madhuri Kango-Singh1,2

1: Department of Biology, University of Dayton, Dayton, OH
2: Integrative Science and Engineering Center, Dayton, OH

Description

Drosophila melanogaster is an ideal genetic model for studying early changes in tumor cells for diseases such as Glioblastoma (GBM) for which there are no current treatment options. A large percent of GBM patients show excess EGFR signaling due to amplification of Epidermal Growth Factor Receptor-VIII (EGFR-VIII), and activation of the phosphatidylinositol 3-kinase (PI3K) pathway. Using mutations to activate the PI3K and EGFR receptors in Drosophila, we have modeled GBM in the fly brain using the GAL4-UAS system.My project involves genetic crosses that will produce larvae with GBM, the dissections of the larval brains, and performing immunohistochemistry to study changes in signaling pathways that promote GBM growth. To do so, I will be studying the early time points to decipher the roles of signaling pathways like MAPK, Hippo and WNT in promoting GBM growth and/or inhibiting cell death. A comparison between our GBM models and experimental controls will provide the initial data for designing further genetic experiments to narrow down specific signaling interactions that affect cell death and proliferation.

Publication Date

4-20-2022

Project Designation

Course Project

Primary Advisor

Madhuri Kango-Singh

Primary Advisor's Department

Biology

Keywords

Stander Symposium project, College of Arts and Sciences

United Nations Sustainable Development Goals

Good Health and Well-Being

Using a Drosophila Glioma Model to Study Signaling Pathways

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