Establishing a Drosophila colon cancer model to study interactions and therapeutic targets of oncogenic pathways
Colorectal cancer (CRC) is the second most common cause of cancer death in the United States, with an estimated 147,950 new cases in 2020. Current treatment varies based on severity of the cancer. If potentially curable, the patient may undergo surgery; in advanced cases, chemotherapy may improve and maintain quality of life. Both treatment options are invasive and target healthy cells as well as cancerous ones. Therefore, it is important to find therapeutic targets for less invasive and more effective treatment. The objective of this project is to develop CRC models in fruit flies to test the role of Hippo and Wnt pathways in gastrointestinal cancer as potential therapeutic targets. To do so, we have (a) developed a CRC model in flies, and (b) tested the levels of Hippo and Wnt pathway activity in this model. Using fly mutants and transgenic flies we have created small patches of cancerous cells in the fly intestine in which have activated oncogenic Ras (mutation RasV12) and dominant negative p53 (mutation UASp53H15N) together with loss of function of APC. This model allows evaluation of multiple genetic combinations (one-, two-, or three- hit models) to evaluate the induced tumor, its growth profile, and the effect of the drug on tumor growth. Here we present our progress with the establishment and analyses of this tumor model.
Madhuri Kango-Singh, Amit Singh
Primary Advisor's Department
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being
"Establishing a Drosophila colon cancer model to study interactions and therapeutic targets of oncogenic pathways" (2022). Stander Symposium Projects. 2441.