Interaction between dorsal patterning gene, dve and Dpp signaling during Drosophila eye development

Interaction between dorsal patterning gene, dve and Dpp signaling during Drosophila eye development

Presenter(s)

Anuradha Venkatakrishnan, Katie Perry, Saba Qureshi, Neha Gogia

Comments

Presentation: 9:00 a.m.-10:15 a.m., Kennedy Union Ballroom

Additional authors:
Saba Qureshi1, Katie Perry1, Anuradha Venkatakrishnan Chimata1, Neha Gogia1, Madhuri Kango-Singh1,2,3,4, Amit Singh1,2,3,4,5

1. Department of Biology, University of Dayton, Dayton, OH
2. Premedical Program, University of Dayton, Dayton, OH
3. Center for Tissue Regeneration & Engineering (TREND), University of Dayton, Dayton, OH
4. Integrative Science and Engineering (ISE), University of Dayton, Dayton, OH
5. Center for Genomic Advocacy (TCGA), Indiana State University, Terre Haute, IN

Description

Morphogens are associated with a multitude of developmental processes, including organ patterning and the control of organ size. During development, axial patterning is crucial for the transition of a monolayer organ primordium into a three-dimensional organ. It involves the delineation of antero-posterior (AP), proximo-distal (PD) and dorso-ventral (DV) axes. During Drosophila eye development, DV axis formation is the first lineage restriction event and is tightly regulated by various signaling cues and transcription factors. Any errors in this process result in developmental birth defects. Previously, we identified defective proventriculus (dve) as a new dorsal eye fate selector or patterning gene. dve regulates eye development by regulating wg, a negative regulator of eye development. Apart from wg, Dpp signaling pathway is important for retinal differentiation. We hypothesize that dve, a transcription factor, regulates the Decapentaplegic (Dpp) signaling pathway, which is comparable to bone morphogenetic protein (BMP) signaling in mammals, during eye development. Here, we explored the interaction between dve with Dpp signaling pathway. We will investigate the role of dve in the dorsal eye and whether dve requires Dpp signaling to specify eye and head fate to form an organ of balanced size and shape. We have utilized the GAL4-UAS system to modulate Dpp signaling in the dve domain. So far, we observe that downregulation of Dpp siggnaling in the dve domain (dve>brk) results in eye suppression while upregulating Dpp levels in dve domain (dve>dpp) results in an enlarged eye field. Additionally, we found that upregulating Dpp in the dve domain reduced Wg expression and conversely, downregulating Dpp in the dve domain resulted in ectopic induction of Wg. This study may have a significant bearing on growth, signaling and patterning defects and help in understanding the etiology behind genetic birth defects in the eye.

Publication Date

4-20-2022

Project Designation

Independent Research

Primary Advisor

Madhuri Kango-Singh, Amit Singh

Primary Advisor's Department

Biology

Keywords

Stander Symposium project, College of Arts and Sciences

United Nations Sustainable Development Goals

Good Health and Well-Being

Interaction between dorsal patterning gene, dve and Dpp signaling during Drosophila eye development

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