Genome-wide screening of miRNA’s in dve cells identifies evolutionary conserved miR-190 regulates eye size by targeting Eyeless/PAX-6

Genome-wide screening of miRNA’s in dve cells identifies evolutionary conserved miR-190 regulates eye size by targeting Eyeless/PAX-6

    Presenter(s)

    Mani Manivannan Subramanian; other author: Amit Singh

    Comments

    Presentation: 10:20-10:40 a.m., Science Center 150

    Description

    Congenital aniridia is caused by mutation(s) in paired-box gene 6 (PAX-6), and is characterized by eye defects manifested in childhood leading to visual impairment. A highly conserved PAX-6 (Drosophila homolog, eyeless, ey), encodes a DNA-binding transcription factor, whose loss-of-function exhibits loss-of-entire-eye or part-of-eye. Surprisingly, molecular genetic basis of Aniridia remains poorly understood. The retinal loss may arise due to dysregulation of miRNAs which plays a pivotal role in regulating genes post transcriptionally. miRNAs are the short hairpin like structure with 20-25bp which modulates the gene expressions post-transcriptionally by binding to 3’UTR of mRNAs. Studies on miRNAs have shown that miRNAs are involved in fine tuning of genes which regulates cell differentiation and proliferation of retinal cells in vertebrates. miRNA serves a vital role in the retina throughout development and in eye diseases. In our study using Drosophila as a model system, importance of miRNAs involved in the development of eye was investigated and in our genome wide screening of miRNAs in dve cells, we have identified miR-190 as our novel candidate in modulating the eye phenotype. Further Eyeless, a target gene of miR-190, was identified by secondary screening through RNAi lines and analyzed for eye phenotypes. Eye fate markers involved in eye development were analyzed in flies expressing miR-190 target gene Eyeless-RNAi. Here, we provide a mechanism of how miR-190 modulates eye phenotype through Eyeless gene, and results from these studies will be discussed in the symposium.

    Publication Date

    4-19-2023

    Project Designation

    Independent Research

    Primary Advisor

    Amit Singh

    Primary Advisor's Department

    Biology

    Keywords

    Stander Symposium, College of Arts and Sciences

    Institutional Learning Goals

    Scholarship

    Genome-wide screening of miRNA’s in dve cells identifies evolutionary conserved miR-190 regulates eye size by targeting Eyeless/PAX-6

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