Regulation of growth and patterning in the developing eye of Drosophilamelanogaster by the Hippo pathway coactivator yorkie and dorsal selectordefective proventriculus

Regulation of growth and patterning in the developing eye of Drosophilamelanogaster by the Hippo pathway coactivator yorkie and dorsal selectordefective proventriculus

    Presenter(s)

    Rohith Basavanahalli Nanjundaiah

    Comments

    Presentation: 10:45-12:00, Kennedy Union Ballroom

    Description

    The developing eye of Drosophila is a well-established model for studyingdevelopmental genetic processes and growth regulation. The developmental geneticnetworks discovered in Drosophila are highly conserved in all animals includinghigher mammals. Our long-term goal is to understand the molecular basis of Dorsal-Ventral patterning and growth in the eye by interactions of the dorsal selector genesand growth regulatory genes. We recently identified defective proventriculus (dve) asa candidate for dorsal-ventral eye patterning that acts as a transcriptional regulator.Gain of function of Dve, results in eye suppression, while loss of function of dve,exhibits dramatic eye enlargement phenotypes which raised an interesting question,whether the dorsal patterning gene dve, apart from its main function of specifyingcells fate, plays a dual role in regulating growth during eye development inDrosophila? We hypothesized that Dve may interact with the Hippo growthregulatory pathway to control patterning and growth of the eye. We tested theinteractions between Hippo pathway and dorsal-ventral patterning using the GAL4-UAS system and MARCM. To investigate whether yki is involved in dve domaingrowth, we used GAL4 drivers in the eye like Dve Gal4 to drive the spatiotemporalexpression of transgenes in the dorsal eye, and Ey-Gal4 and GMR-Gal4 to test theeffects of Dve and Yki modulations before and after MF formation in the larval eyeantennal disc. Initial data suggests genetic interactions between Hippo and DVpatterning genes are important in early eye development. We have tested wingless(wg) a known and conserved Hippo downstream target for Dve and Yki mediatedeffects using reporter assays, clonal analysis

    Publication Date

    4-17-2024

    Project Designation

    Independent Research

    Primary Advisor

    Madhuri Kango-Singh, Amit Singh

    Primary Advisor's Department

    Biology

    Keywords

    Stander Symposium, College of Arts and Sciences

    Regulation of growth and patterning in the developing eye of Drosophilamelanogaster by the Hippo pathway coactivator yorkie and dorsal selectordefective proventriculus

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