Presenter(s)

Kelechi Richie Okere

Comments

Presentation: 1:15-2:30, Kennedy Union Ballroom

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Description

Influenza A virus (IAV) is an enveloped, negative-sense, single-stranded, segmented RNA virus.It is one of the highly infectious viruses that resulted in at least 14 or more viral pandemics inhistory, including influenza virus pandemics in 1918, 1957, 1968, 1977, 2009, and 2013.Inactivated whole virus and live attenuated virus vaccines effectively control seasonal influenzavirus infections. Subunit vaccines are considered safer than inactivated or live attenuated virusvaccines, especially among elderly people. The current experiment in the Immunology lab (BIO496L) was designed to prepare a subunit vaccine for IAV using its nucleoprotein (NP) andanalyze for vaccine response in rats against the virus. IAV A-NP, strain A/PR/8/35 was mixedwith Freund's complete adjuvant and injected into the rats (Sprague Dawley) subcutaneouslyaccording to the University of Dayton IACUC protocol. A booster dose was given after oneweek of primary vaccination and blood was collected after two weeks of booster vaccination.The serum was separated and analyzed for IAV A nucleoprotein (NP) specific antibodies using aprecipitation test, Enzyme-Linked Immunosorbent Assay (ELISA), and hemagglutination (HA)/hemagglutination inhibition (HI) assays. Results showed IAV A nucleoprotein (NP) specificantibodies in the precipitation test, while ELISA showed a 10.74±0.71 increase in antigen-specific antibodies in the rat serum vaccinated with the subunit vaccine, whereas HA/HI assayshowed 80 HI units for influenza virus A neutralizing antibodies. Overall, the current experimentshowed that the subunit vaccine prepared in the lab induced a protective immune responseagainst the influenza A virus.

Publication Date

4-17-2024

Project Designation

Course Project 202380 BIO 496L 01

Primary Advisor

Tooba Shafeeque Ahmed Momin, Mrigendra Rajput

Primary Advisor's Department

Biology

Keywords

Stander Symposium, College of Arts and Sciences

Preparing subunit vaccine for Influenza A virus and measuring its immune response in rats

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