JAK STAT Signaling regulates defective proventriculus (dve) to determine dorso-ventral patterning in Drosophila eye.

JAK STAT Signaling regulates defective proventriculus (dve) to determine dorso-ventral patterning in Drosophila eye.

    Presenter(s)

    Sunanda Yogi; other researchers: Akansha Raj, Anuradha Venkatakrishnan Chimata, Madhuri Kango-Singh, Amit Singh

    Comments

    Presentation: 1:15-2:30, Kennedy Union Ballroom

    Description

    Axial patterning is the fundamental process of organogenesis, which entails delineation of three distinct axes: Antero-Posterior (AP), Dorso-Ventral (DV) and Proximo-Distal (PD) axes. Any impairment in the axis formation may lead to developmental birth defects in humans and therefore, getting insight of the mechanism of axis determination is crucial for better understanding of organogenesis. In Drosophila eye model, DV patterning is the primary lineage restriction event. A new member of DV patterning gene, defective proventriculus (dve, a Homeobox gene), an ortholog of SATB homeobox 1 (special AT-rich sequence binding protein 1) has been identified which acts downstream of a GATA-1 transcription factor pannier (pr), and upstream of wingless (wg) in the dorsal gene hierarchy. Unpaired (Upd), a long-range secreted ligand for JAK STAT pathway, is known to promote eye development by negatively regulating Wg expression. Here we present that Upd interacts with dve, to regulate the patterning and growth of the developing Drosophila eye. We found that activation of JAK STAT pathway in dve expression domain results in dorsal eye enlargement and downregulation of Wg expression whereas its inactivation in Dve domain results in eye suppression phenotype and upregulating Wg expression. Our data strongly imply that Upd plays a crucial role in defining the functional domain of Dve during DV axis formation of developing eye. We will present the complex interactions between these two highly conserved pathways, viz., JAK-STAT and dorsal eye fate selectors in growth and patterning of the eye.

    Publication Date

    4-17-2024

    Project Designation

    Graduate Research

    Primary Advisor

    Madhuri Kango-Singh, Amit Singh

    Primary Advisor's Department

    Biology

    Keywords

    Stander Symposium, College of Arts and Sciences

    JAK STAT Signaling regulates defective proventriculus (dve) to determine dorso-ventral patterning in Drosophila eye.

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