Presenter(s)

    Caroline Wise

    Download

    Download Project (139 KB)

    Description

    In response to the current global energy crisis, biofuels have become a viable renewable energy solution and require a carbohydrate source to begin their production. One such carbohydrate source option is biomass, which is comprised of complex sugars that can be broken down into simple sugars and then fermented for the production of bioethanol. The bacterium called Caldicellulosiruptor saccharolyticus contains many glycoside hydrolase enzymes that have the potential for metabolizing the complex sugars in several carbohydrate sources, including those in biomass. This project is focused on the cloning of the Csac_2410 gene from C. saccharolyticus, expression of the gene as a protein, purification of the protein, and biochemical characterization of the protein. The biochemical characterization determines the substrate specificity, pH optima and temperature optima of Csac_2410, and the results are used to determine the effectiveness of Csac_2410 in metabolizing complex sugars for the upstream processing of biofuels.

    Publication Date

    4-9-2014

    Project Designation

    Honors Thesis

    Primary Advisor

    Donald A. Comfort

    Primary Advisor's Department

    Chemical and Materials Engineering

    Keywords

    Stander Symposium project

    Disciplines

    Arts and Humanities | Business | Education | Engineering | Life Sciences | Medicine and Health Sciences | Physical Sciences and Mathematics | Social and Behavioral Sciences

    Purification and Biochemical Characterization of a Xylanolytic Glycoside Hydrolase from Caldicellulosiruptor saccharolyticus

    Share

    COinS