Honors Theses
Advisor
Shawn Swavey, Ph.D.
Department
Chemistry
Publication Date
4-1-2023
Document Type
Honors Thesis
Abstract
A new single molecule 4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) dye designed to conjugate to the peripheral amines of proteins has been synthesized. Detailed synthesis involving a new and simple two step technique is presented. Synthesis of the carboxylic acid substituted BODIPY dye is characterized by absorption and emission spectroscopy. The new dye is reacted with N- hydroxysuccinimide to give a reactive BODIPY dye toward amine groups of bovine serum albumin. The BODIPY-N-succinimide ester was evaluated by absorption and emission spectroscopy. Covalent attachment of the BODIPY-NHS-ester with bovine serum albumin was conducted in an eppendorf tube in a phosphate buffer at a pH of 7.0. Purification by column chromatography and evaluation of the solution suggests a degree of labeling of only 50% of the bovine serum albumin.
Permission Statement
This item is protected by copyright law (Title 17, U.S. Code) and may only be used for noncommercial, educational, and scholarly purposes.
Keywords
Undergraduate research
eCommons Citation
Hess, Amy, "Boron Dipyrromethene (BODIPY) Dye Covalently Bound to Bovine Serum Albumin (BSA)" (2023). Honors Theses. 401.
https://ecommons.udayton.edu/uhp_theses/401
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