Honors Theses
Advisor
Justin Biffinger, Ph.D.
Department
Chemistry
Publication Date
4-1-2023
Document Type
Honors Thesis
Abstract
Esters and amides are the most versatile functional groups in nature and in plastics today. Understanding the key temporal events in the biodegradation and susceptibility of polymers and plastics (e.g. polyester and polyester polyurethanes) to hydrolytic enzymes is important to the sustainable development of our modern society. I will present the isolation, identification, and classification of an environmental strain of Naganishia albida found on polymer coatings inside of aircraft. To better understand the capability of hydrolytic enzymes to degrade plastics we isolated, identified, and classified an environmental strain of Naganishia albida found on polymer coatings inside an aircraft. We utilized cultivation conditions that induced the secretion of several hydrolytic proteins (between 30 kDa and 140 kDa) from N. albida that were identified via LC-MS. We also isolated the hydrolytic proteins from cellular supernatants using size exclusion chromatography, and performed soluble colorimetric esterase assays and polyester polyurethane coating degradation experiments. Finally we showed comparisons between the hydrolytic proteins produced by N. albida and another polymer degrading yeast, Papiliotrema laurentii.
Permission Statement
This item is protected by copyright law (Title 17, U.S. Code) and may only be used for noncommercial, educational, and scholarly purposes.
Keywords
Undergraduate research
eCommons Citation
Ove, Clarissa A., "Identification and Comparison of Hydrolases Secreted from Naganishia albida to Papiliotrema laurentii and their activity toward natural and synthetic polyesters" (2023). Honors Theses. 414.
https://ecommons.udayton.edu/uhp_theses/414
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