Honors Theses


Matthew E. Lopper



Publication Date

Spring 4-2015

Document Type

Honors Thesis


Deinococcus radiodurans is a species of bacteria that has sparked a lot of interest since its discovery due to its incredible resistance to ionizing radiation. When exposed to ionizing radiation the genome of D. radiodurans will sustain over one hundred double stranded breaks. D. radiodurans demonstrates the ability to repair its genome and restart replication after sustaining, typically lethal, DNA damage. This project examined the mechanism of replication restart in D. radiodurans by investigating primosome protein PriA interacting with replicative helicase DnaB and single stranded binding protein. Many different types of gel electrophoresis were employed to investigate potential protein complex formations between D. radiodurans PriA and DnaB. Native agarose gel electrophoresis successfully revealed an interaction between D. radiodurans PriA and DnaB. Results in this work indicate that D. radiodurans PriA can interact with DnaB.

Permission Statement

This item is protected by copyright law (Title 17, U.S. Code) and may only be used for noncommercial, educational, and scholarly purposes.


Undergraduate research


Chemistry | Physical Sciences and Mathematics

Included in

Chemistry Commons