Document Type
Article
Publication Date
4-2010
Publication Source
Tissue Engineering Part C Methods
Abstract
This study investigated the encapsulation of newt iris pigment epithelial cells (PECs), which have the ability to regenerate a lens by trans-differentiation in vivo, within a biodegradable hydrogel of oligo(poly(ethylene glycol) fumarate) crosslinked with poly(ethylene glycol)-diacrylate. Hydrogel beads of initial diameter of 1mm were fabricated by a molding technique. The swelling ratio and degradation rate of the hydrogel beads decreased with increasing crosslinking ratios. Confocal microscopy confirmed the cytocompatibility of crosslinking hydrogel formulations as evidenced by the viability of an encapsulated model cell line within a crosslinked hydrogel bead. Hydrogel beads encapsulating iris PECs were also implanted into lentectomized newts in vivo; histological evaluation of explants after 30 days revealed a regenerated lens, thus demonstrating that the presence of degrading hydrogel did not adversely affect lens regeneration. The results of this study suggest the potential of a method for lens regeneration involving oligo(poly(ethylene glycol) fumarate) hydrogels for iris PEC encapsulation and transplantation.
ISBN/ISSN
261-267
Document Version
Published Version
Copyright
This is a copy of an article published in Tissue Engineering Part C Methods © 2010, copyright Mary Ann Liebert, Inc.
Publisher
Mary Ann Liebert
Volume
16
Peer Reviewed
yes
Issue
2
Keywords
Mary Ann Liebert Inc, Article, Growth-Factor Delivery, Stem-Cells, In-Vitro, Chondrogenic Differentiation, Osteogenic Differentiation, Composites, Scaffolds
eCommons Citation
Zhang, Mimi W.; Park, Hansoo; Guo, Xuan; Nakamura, Kenta; Raphael, Robert M.; Kasper, F. Kurtis; Mikos, Antonios G.; and Tsonis, Panagiotis A., "Adapting Biodegradable Oligo(Poly(Ethylene Glycol) Fumarate) Hydrogels for Pigment Epithelial Cell Encapsulation and Lens Regeneration" (2010). Biology Faculty Publications. 11.
https://ecommons.udayton.edu/bio_fac_pub/11
