Molecular genetic dissection of a transcription factor Defective proventriculus in growth versus cell fate decisions

Authors

Presenter(s)

Anuradha Chimata Venkatakrishnan

Comments

Presentation: 1:15-2:30, Kennedy Union Ballroom

Files

Description

Defective proventriculus, a homeodomain containing transcription factor acts as a dorsal fate selector during Drosophila eye development. It is highly conserved across species and its human ortholog SATB1 is highly upregulated in cancer. Earlier, we have shown that dve is crucial to establish dorsal fate which is required to promote growth and cell fate decisions. Furthermore, the dve expressing cells (~150-200) are responsible for expression of a morphogen wingless (wg)/Wnt in the developing eye. These dve expressing cells provide an inductive signal to determine eye vs head fate specification. Since dve is involved in growth regulation and cell fate decisions, we wanted to dissect these two independent functions during eye development. We employed structure-function analysis because dve has two isoforms and the Dve protein has multiple functional domains such as ULD domain, two Hox domains and a PPP4R2 domain. We hypothesized that different domains of Dve might be involved in regulating different functions. We present our results from studies on the role of these domain(s) on eye development and regulation of Wg expression in the developing eye. Here, we present results from individual functional domain(s) of Dve, combination of domain(s) and chimeras with SATB1. SATB1, human ortholog of dve, has similar domains and such studies can shed light on how SATB1 might function in diseases.

Publication Date

4-17-2024

Project Designation

Graduate Research

Primary Advisor

Madhuri Kango-Singh, Amit Singh

Primary Advisor's Department

Biology

Keywords

Stander Symposium, College of Arts and Sciences

Recommended Citation

"Molecular genetic dissection of a transcription factor Defective proventriculus in growth versus cell fate decisions" (2024). Stander Symposium Projects. 3594.
https://ecommons.udayton.edu/stander_posters/3594