Dve interacting partner chb role in microtubule dynamics during eye development

Dve interacting partner chb role in microtubule dynamics during eye development

Presenter(s)

Sunanda Yogi

Comments

Presentation: 1:15-2:30, Kennedy Union Ballroom

Description

Chromosome bows (chb)/Mast/Orbit is required for bipolar mitotic spindle organization at the kinetochore. This is an important microtubule plus end tracking protein functions in maintaining the microtubule dynamics. It is evolutionary conserved and the human ortholog is cytoplasmic linker associated protein 1 (CLASP1) which plays crucial role in microtubule distribution and stability during cell cycle and has been shown to impact neurofibrillary tangle formation in tauopathies. To mechanistically understand, how chb regulate microtubule orientation? We are utilizing Drosophila, a genetic tractable model system with various developmental stages and looked at its genetic interactome, where has been shown that chb is being suppressed by defective proventriculus (Dve). It is a K50 homeodomain transcription factor required for the cell type specification and expressed in the dorsal head vertex region of eye-antennal imaginal disc of Drosophila. We hypothesize that if chb expression is regulated by dve which results in altered kinetochore assembly, when we knockdown the expression of chb using RNAi (RNA interference) in the eye, it gives us small and necrotic regions in the proper ommatidium arrangement of the eye. We are further interested into looking at what happens to dve expression and wg which is negative regulator of eye development. This will further illustrate on key partners involved in the cellular assembly of microtubules.

Publication Date

4-17-2024

Project Designation

Graduate Research

Primary Advisor

Madhuri Kango-Singh, Amit Singh

Primary Advisor's Department

Biology

Keywords

Stander Symposium, College of Arts and Sciences

Dve interacting partner chb role in microtubule dynamics during eye development

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