Kristina-Lerin Kealoha Linares, Jason Andrew Tornes
Adequate sleep is important for good health. College students who are involved in extracurricular activities and working jobs have commitments that impact their ability to get adequate sleep. According to the National Sleep Foundation, young adults ages 18-25 years old need between 7-9 hours of sleep every night (Singh et al., 2021). A study on college students identified an average sleep time of 6.99 hours after interviewing 7,626 college students (Becker et al., 2018). “Short-term consequences of sleep disruption include increased stress responsivity; somatic problems; reduced quality of life (QoL); emotional distress; mood disorders and other mental health problems; cognition, memory, and performance deficits; and behavior problems in otherwise healthy individuals,” (Medic et al 2017). Our research focuses on the sleep habits of students at the University of Dayton. We surveyed students to gather information including their year of study, the typical hours of sleep they receive, and if they currently take any sleep aids (i.e. melatonin, Benadryl, etc.). The information collected was then statistically analyzed using the ANOVA (Analysis of Variance) statistical test to determine if there is a significant correlation between the sleep habits of UD students and their year of study. The purpose of this poster is to assess the sleep habits of UD students and bring awareness to the importance of getting adequate sleep for overall well-being. Practical tips for improving sleep habits will also be included in our presentation.
James Treat Huff, Daniel P. Hughes, Collin Timothy Schmid, Luke Collin Meissner
Research question: How does the Three O'Clock Prayer sculpture communicate and construct religious identity at UD?
The tiny things that restore the prairie: the impacts of selective planting and soil amendments on microbial activity during the restoration of a degraded agriculture field in the American Midwest
Ellie Adriana Wasilewski
Many forests and prairies have been cleared for agricultural purposes, resulting in ecological degradation and altered biological communities. In recent years, agricultural land has been left fallow and is ripe for restoration back to native ecosystems. Abandoned agriculture results in a deficiency of nitrogen and carbon, changing the microbial activity in the soil. Restoring microbial community function can improve nutrient cycling and enhance plant species establishment during restoration. Restoration efforts, including soil amendments and selective planting are used to increase the nutrient content of the soil, and can improve microbial function. It has been suggested that these efforts may accelerate ecological succession. We assessed plant cover and enzyme activities of peroxidase, phenol oxidase, and beta glucosidases one year following the addition of leaf compost, whole soil transplant, and selective seeding on a 20 acre post-agricultural field. Here, we report the response of soil microorganism function, via enzyme activity, to planted seeds and soil amendments one year after restoration implementation. Phenol oxidase, peroxidase, and beta glucosidase have lower activity where there are more grass species. When there is a high presence of grass species, more niche space is taken up and more nitrogen and carbon is being used by the grasses leaving less available for microorganisms. Beta glucosidase is shown to be higher with whole soil treatment. Whole soil amendments could have introduced new nutrients that were previously absent and microbes that could uptake nutrients more readily than the microbes present in the degraded field. Peroxidase activity is higher where there was low diversity and high legume concentration and lowest in low richness low legume and high richness high legume concentrations. Understanding how microorganism activities can be altered using soil amendments and selective planting can help restorationists support healthy ecological succession.
Visual Arts majors in the Department of Art and Design will present research and analysis of their individual journey culminating in creative self-directed artistic projects. Each student project is unique and reflects their selected vocations in the visual arts determined by a review of professional practices, standards and activities across related disciplines.
Meghan Elizabeth Leinhauser
The purpose of this study is to analyze the Missing and Murdered Indigenous Women, Girls, and Two-Spirited People (MMIWG2S) social movement via content analysis and interviews. The study includes analyzing videos posted to the social media platform, TikTok, and interviews with a collection of users who post videos about MMIWG2S. TikTok uses a unique algorithm to tailor users’ feeds based on the types of videos with which they watch and interact. This allows for the creation of an online community that shares common interests with one another, especially in a time where communication methods have changed significantly. Analyzing videos related to the movement allows for emergent themes and trends to be found within the posts. Doing so aids in understanding what these individuals want the general public to know about a movement that means so much to them. The subsequent interviews allow for a deeper comprehension of the role social media, specifically TikTok, plays in responding to the MMIWG2S social movement.
Matthew Thomas Coyne, Christina J. Schroedle
According to a study conducted in 2017, about 792 million people in the world have a diagnosed mental health issue, and specifically approximately 284 million people are diagnosed with anxiety. Anxiety can create barriers in people’s life that can have major effects on someone’s day to day activities. One specific form of anxiety is related to health, and this type of anxiety is seen to increase in the hospital setting. There have been many studies done about how anxiety levels in nurses and physicians impact the care that is given to the patients and the results from these high anxiety levels (Chen et al. 2022). We want to dive into the other end of the issue; how a patient's anxiety level impacts their own care. This study will give us qualitative data and there will be a wide variety of participants. Meaning that we will be speaking to patients on all different floors of the hospital, such as oncology, pulmonary, burn units about different factors within a hospital setting that impact their anxiety levels. The goal of this study is to see how the patient’s mental health is being treated in the hospital.We believe that mental and physical health are extremely connected, and we will be trying to find strong correlation between the two factors throughout the study.
Title: Identification of novel genes responsible for a rapidly evolving fruit fly trait by gain and loss of function approaches
Jada H. Brown, Gavin M. Christy
Two long-standing goals of geneticist are to understand how genes are usedduring an organism’s development to make morphological traits, and how genes changeto facilitate the origins, modifications, and losses of these traits. Two powerfulapproaches to identify the critical genes are through gain and loss of functionexperiments. The former experiments look at the effects when the expression of genes isinduced in new cellular places, at new times in life, and/or at elevated levels ofexpression. The latter experiments cause the absence of expression for genes from theirnormal places, times, and/or levels of expression. While many methods have beendevised to accomplish such experiments, these methods are generally too cumbersome toapply to tens, hundreds, or even thousands of genes. In recent years, scientists have foundways to use RNA-interference and CRISPER/Cas9 gene editing to cause both gain andloss of function mutations for specific genes. These methods are being combined withresources for the fruit fly species Drosophila (D.) melanogaster to target a steadilyincreasing number of this species’ genes. Our research project applies these approachesand genetic tools to cause gain and loss of function mutations in genes suspected to play arole in the development and evolution of the male-specific pigmentation of the D.melanogaster abdomen. Any gene whose altered function distorts this pigmentationfeature will become a target of future studies to tease out the mechanisms of genefunction and evolution.
Harmful Algal Blooms (HABs) have become more prevalent in recent decades due to increased anthropogenic nutrient input and warmer water temperatures associated with climate change. Nutrient (nitrogen) inputs include loading from the expansion of urban centers (wastewater discharge) and agricultural intensification (inorganic fertilizers and animal waste). In this study, water samples from Saginaw Bay and its watershed were analyzed using a combination of water chemistry, stable water isotopes (δD and δ18O), and nitrate stable isotopes (δ15N and δ18O). The purpose of this study was to evaluate sources, processes, and spatial patterns of nitrate in Saginaw Bay and its watershed causing HABs. We collected water samples from 23 streams and rivers across the watershed and 9 sites from the Bay. The result shows that: 1) the mean nitrate concentration tends to be higher (1.79 mg/l, nitrate-N) in streams that drain from areas with high agricultural land use and concentrated animal feeding operations, 2) the mean nitrate concentration in the Saginaw Bay is relatively low (0.41 mg/l, nitrate-N) and uniformly distributed throughout the water column, 3) the average δ15N value for river samples was 10.69‰ and the average δ18O value was 3.48‰. The dual-isotope analysis result suggests that manure and septic waste are the main sources of contaminants in the watershed. The relative nitrate contribution from manure and septic waste ranged from 35% to 86%. The second-largest nitrate source was typically inorganic fertilizer and its contribution ranged from 3% to 49%. In addition, nitrification sources contribute 7% to 15% of nitrate contamination in the watershed. Ultimately, the outcome of the study provides vital information for effective nutrient management in the Saginaw Bay watershed to prevent future expansion of HABs and further deterioration of water quality in the Great Lakes Region.
Trashy! A multi-season study of anthropogenic and organic litter in riparian areas along the Great Miami River.
Allyson A. Allen, Emily Marie Berkshire, Charles M. Brockman, Herbert K. Burroughs, Pepper Julia Cantwell, Olivia P. Fenner, Grace A. Janszen, Catherine G. Landry, Morgan E. Mieland, Miles Ellison Muratore, Emerson R. Phares, Megan Ann Poole, Kendall E. Ridgley, Michaela M. Rogers, Rianna Soltis, Adelaide Lorraine Starks, Amanda N. Thieneman, Valerie Nicole Thurston, Cheryl Vargas, Juliana Marie Vollmer
Rivers act as conduits of both anthropogenic and organic litter that eventually reach oceans. Few studies have investigated how anthropogenic litter and organic litter vary along gradients from the river’s edge into riparian ecosystems, if anthropogenic and organic litter are spatially coupled with one another, and how anthropogenic litter composition varies across riparian areas. The ecosystem ecology lab (BIO 459L) has been studying riparian litter for the last couple of years along the Great Miami River. We set up transects to look at anthropogenic and organic litter from the river’s edge, and every two meters inland up to 10m. We found evidence that anthropogenic litter and organic litter are spatially coupled, and that the composition of anthropogenic litter changes along a gradient from the river’s edge to inside riparian areas. This type of information can help land managers understand how to manage litter in riparian areas of the Miami Valley.
Benjamin Joseph Crawford, Gillian R. Leone
This panel includes two presentations and a moderated discussion:Paper 1 - “Political Passion, Engagement, and Trust—and Trump” by Ben CrawfordPaper 2 - “’My Body, My Choice’: Abortion Rights, Public Health, and Trust during the COVID-19 Pandemic” by Gillian LeoneThe period since the advent of the COVID-19 pandemic in early 2020 has been unique in terms of trust (or mistrust) in government, science, and public health experts. The pandemic arrived during a particularly contentious time in our history, with a tumultuous and contested presidential election occurring in its midst. These two papers use 2020 American National Election Study data to explore topics related to trust in government and science, including the determinants of political trust, passion, and engagement; voting behavior; and attitudes on abortion rights, public health mandates, and vaccines. Ben’s paper began as an undergraduate Capstone project and continued as a graduate independent study, while Gillian’s paper began as a collective in-class project during POL 300 Religion and Politics in Fall 2021. Ultimately, the papers reinforce the democratic values of public trust, engagement, and respectful dialogue in society to weather political and medical crises (and crises of conscience).
UD Campus Sustainability: Report from the Fall 2021 Campus Survey on Beliefs, Knowledge, and Actions.
Cassie Marie Austin, Darius Beckham, Benjamin Joseph Crawford, Nathaniel Steven Duran, Mason E. Gordon, Patricia L. Thomas, Dimitrios Anthony Toliopoulos
Students conducted a campus-wide survey during Fall 2021 as part of UD's submission for The Sustainability Tracking, Assessment & Rating System (STARS) report. We examine the beliefs, knowledge, and actions of the UD campus community around issues of sustainability.
Noah R. Bischoff, Kaila Colacarro, Brendan P. Connaughton, James D. Cook, Matthew Ward Cramsie, Carolyn M. Duvic, Kobe Curtis Elvis, Grace K. Fuller, Claire Eliza McLaughlin, Nicholas J. Miozzi, James Gillis Moag, Sofia Grace Perez, Catherine Drew Ridenour, Samuel Bernard Seltzer, Anthony Joseph Semerano, Andrew Joseph Siciliano, Aaron James Spieles, Jacob Nicholas Ward, Zachary Allen Zienka
Superhero movies are currently the dominant film form in U.S. cinema, as demonstrated by two decades of box office top tens populated by characters from the pages of Marvel and DC Comics, and other studios and movie franchises scrambling to replicate their success any way they can. How did we get here, and what is the effect of these superhero films on American film culture and global popular culture? The presentations for this panel will explore the superhero genre, considering how their films adhere to or defy genre conventions, challenge predominant American ideologies, and function as works of political economy.
Andrew William Bartsch, Eric Hayes Litterer, Kahra Gikanga Loding, Elizabeth E. Miles-Flynn, Leonard Patrick O'Connor, Lizbeth Marie Ramos-Ortiz, Samantha S. Trajcevski
Aligned with the University of Dayton (UD)’s vision for the common good and the Characteristics of Marianist Universities (https://marianistuniversities.org), UD pledged commitment to the Laudato si Action Platform (LSAP), (https://laudatosiactionplatform.org) journey in October 2021. The LSAP integrates the UN Sustainable Development Goals (UNSDGs), and Catholic social teaching as outlined in Pope Francis’ encyclical Laudato si’. Universities are one of the seven major LSAP sectors (https://laudatosiuniversities.com), giving students a unique opportunity to impact change both locally and globally. UD has many student-led organizations dedicated to environmental sustainability, human rights, Marianist community, social justice, advocacy, and more. While many groups engage in outreach, advocacy and activism, the collective impact could be furthered by greater sharing of resources, ideas, and skills around common goals. This interactive session gathers UD student leaders and attendees to share perspectives on how UD can best engage the LSAP goals (caring for the earth, aiding the marginalized, ecological education, economics and spirituality; sustainable living, and community empowerment.) Brief background presentations on the LSAP and the new Marianist Family Encounter Project will be followed by organization leaders sharing how their group’s missions are already achieving aspects of the LSAP goals. In breakout groups, all participants will explore the various ways they can contribute to accomplishing these goals, as well as building upon campus initiatives. They will also share experiences of successful collaboration and envision how student, faculty, and staff collaboration around LSAP goals could create greater collective impact in all aspects of university life and in UD’s relationship with the greater Dayton community. Students will be called to action to create a unifying body to promote coordination between students, faculty, and staff in pursuit of the LSAP goals to amplify UD’s impact on our campus, in our Marianist family, church, and world.
Undergraduate Campus Involvement and Perceptions of On-Campus Safety: Applying Durkheim’s Theory of Social Integration to the University Environment
Rachel M. Veneman
The purpose of this study is to identify the relationship between the social involvement of University of Dayton undergraduate students and their perceptions of safety on campus. The study hypothesizes that students more involved on campus will have higher perceptions of safety and a lower fear of crime on campus. Previous research shows that social involvement in groups aids in the social integration of an individual into a community. Perceptions of safety and the level of social integration are linked by studies of various communities, but research has overlooked how this plays out in the setting of a college campus with unique involvement opportunities and experiences of crime. Variables such as gender and race are expected to reflect in perceptions of safety, as previous literature shows women and racial minorities tend to have lower perceptions of safety and higher fear of victimization of crime due to various factors besides social involvement. This study employs a survey sent to undergraduate on-campus residents living in university-owned housing that asks about their demographics, on-campus involvement, and perceptions of safety in various ways. The survey was conducted via Qualtrics and retrieved responses anonymously. Responses were analyzed using quantitative data analysis through SPSS. The results identify trends of student safety perceptions based on involvement as well as various demographic factors.
One in five women and one in sixteen men experience sexual assault during college. Sexual assault rates increase for first-year students transitioning into a new environment. Institutions address this issue by implementing consent education. However, empirical assessments on college students’ behavior and attitudes are scarce. To address this gap, the current study used a exploratory case study analysis to examine in-depth the impact of one consent education program on first-year college students’ beliefs, attitudes, and experiences at a Midwestern University. The case study employed document reviews, 20 pre-and post-surveys, and two semi-structured interviews. The analyses included direct interpretation of document reviews, paired sample t-test of pre- and post-surveys, and theme analysis of the interviews. Preliminary findings show that the program defines consent as mutual and verbal agreement between all parties and the role alcohol plays in complicating consent, students’ apprehension to practicing consent, and the impact of social settings on one’s sense of belonging. Policy implications for the University’s approach to sexual consent education will be addressed.
Prajakta D. Deshpande, Esther Lu Lu Seng Labya
Cell size plays an important role in the cellular processes and function of a cell. Hence cell size and growth need to be maintained properly. Gene regulation plays an important role in regulating gene expression of different cellular processes like apoptosis, cell growth, etc. MicroRNAs (miRNAs), small single-stranded RNAs, regulate gene expression post-transcriptionally by binding to the 3’untranslated region of their target messenger RNAs (mRNAs), degrading their target and hence silencing their gene expression. Our lab has identified that miRNA-277 (miR-277) inhibits apoptosis in the Drosophila eye model. We employed the Gal4-UAS system in Drosophila melanogaster to misexpress head involution defective (hid), a pro-apoptotic factor, within the developing fly retina, exhibits reduced eye phenotype. Hid expression is regulated during embryogenesis, larval development, and metamorphosis. Hid is known to be regulated by the Hippo pathway, miRNAs, etc. Our lab found that gain-of-function of miR-277 in the background of GMR>hid rescues the reduced eye phenotype. Furthermore, hid plays a role in programmed cell death (PCD) during pupal development by removing extra-interommatidial cells. The previous study shows that in the hippo (hpo) mutants, hid transcript levels were reduced in interommatidial cells and failed to undergo PCD. The question remains unanswered how hpo modulates hid expression during pupal development. We hypothesize that miR-277 can provide a link since miR-277 inhibits hid mRNA expression. Furthermore, our preliminary data suggest that the gain-of-function of miR-277 in the GMR>hpo background rescues Hpo-mediated cell death. Our working model suggests that miR-277 regulates cell growth by modulating Hippo pathway.
Drosophila melanogaster is an ideal genetic model for studying early changes in tumor cells for diseases such as Glioblastoma (GBM) for which there are no current treatment options. A large percent of GBM patients show excess EGFR signaling due to amplification of Epidermal Growth Factor Receptor-VIII (EGFR-VIII), and activation of the phosphatidylinositol 3-kinase (PI3K) pathway. Using mutations to activate the PI3K and EGFR receptors in Drosophila, we have modeled GBM in the fly brain using the GAL4-UAS system.My project involves genetic crosses that will produce larvae with GBM, the dissections of the larval brains, and performing immunohistochemistry to study changes in signaling pathways that promote GBM growth. To do so, I will be studying the early time points to decipher the roles of signaling pathways like MAPK, Hippo and WNT in promoting GBM growth and/or inhibiting cell death. A comparison between our GBM models and experimental controls will provide the initial data for designing further genetic experiments to narrow down specific signaling interactions that affect cell death and proliferation.
Using a New Conditional Knock-out Mouse Model to Investigate Novel Calcium Signaling Pathways in the Brain
Aikaterini Britzolaki, Ben Klocke
Attention-deficit hyperactivity disorder (ADHD) affects over 8% of individuals in the US, according to the NIH. In addition to impaired attention and locomotor hyperactivity, symptoms often include enhanced impulsivity and memory deficits. The etiology of ADHD is elusive, but one proposed mechanism behind this and many other brain disorders is the dysregulation of intracellular calcium (Ca2+) homeostasis. Ca2+ is a critical second messenger for a constellation of cellular processes, including cell proliferation and death, metabolism, and gene expression. Naturally, neuronal Ca2+ signaling is vital in processes such as long-term potentiation (LTP) and synaptic transmission, among others. Interestingly, our lab has identified a new calcium-handling regulator to be expressed in the mouse brain, and that global genetic ablation of this gene in mice results in the manifestation of an ADHD-like behavioral phenotype. Our lab has recently generated a novel conditional knockout (cKO) mouse model of this gene, that also exhibits locomotor hyperactivity, a cardinal ADHD-like behavior. Further, we have employed the 5-choice serial reaction time task (5-CSRTT), a state-of-the-art behavioral paradigm to assess attentional capacity and impulsivity, two other core symptoms of ADHD. Interestingly, preliminary data from our lab show that the cKO mice exhibit impaired attention in this paradigm. Altogether, these findings strongly support a novel role for this gene in the manifestation of ADHD-like behaviors.
Using in silico and in vivo approaches to reveal the gene regulatory network responsible for the development and evolution of a fruit fly trait
Matthew Dennis Spangler, Michael Weinstein
Animal traits develop through the expressions and actions of numerous genes that comprise a gene regulatory network (GRN). For each GRN, its underlying patterns of expression are controlled by combinations of transcription factor proteins interacting with binding site sequences within cis-regulatory elements (CREs). These interactions drive cell-type and developmental stage-specific transcriptional activation or repression. Function altering mutations in CREs are suspected to be a common source of phenotypic variation within a species, and for the origin, modifications, and losses of traits that differentiate species. Unfortunately, for any trait and its generative GRN, finding CREs remains a slow and resource intensive endeavor. In this study, we sought to better understand the GRN responsible for the male-specific (dimorphic) pattern of abdomen pigmentation of Drosophila (D.) melanogaster fruit flies. This pigmentation trait is a leading model for evolution and development (evo-devo) as variation exists within this species, and between related species. We used an in silico approach to identify predicted CREs (pCREs) that control novel genes within this GRN. Through in vivo assays we demonstrated that many pCREs activate expression in the fruit fly abdomen at the correct developmental stage. Through CRISPR/Cas9 genome editing, we aim to demonstratewhether some of these pCREs are necessary for the D. melanogaster trait, and which gene’s expression they regulate. By reporter transgene assays, we will compare the CRE activity of related sequences from fruit fly species with the derived (dimorphic) and ancestral (monomorphic) pigmentation. This will reveal whether the CRE activities are old and conserved, or modern and perhaps represent some of the GRN modifications responsible for the dimorphic trait. Ultimately, this work will expand the understanding of a leading evo-devo model trait, and perhaps demonstrate an experimental framework by which animal trait evolution can be routinely studied.
Olivia A. Hervey, Alexia Catherine Montino
Trauma among juveniles is a prevalent issue within the juvenile justice system. Juvenile diversion programs may play a role in addressing this trauma. However, juvenile diversion programs use different therapeutic approaches raising questions as to how they might identify trauma. The current study aimed to understand how professionals within a Midwestern juvenile diversion program believe they identify trauma amongst their clients. Using a phenomenological approach, four semi-structured interviews using purposive non-probability sampling were conducted. The analyses included transcribing the interviews verbatim, memoing, initial coding, categories, and thematic analyses. An inter-rater reliability check is performed through a comparison of categories between interviews and the two interviewers. Preliminary results indicate that staff within the diversion program relies heavily on assessment tools to assist with the trauma identification but have not established an internal check within the program. A discussion of these results, implications, limitations and future directions will be discussed.
Connor Brooks Hagan, Nicholas James Patritti, Ian Rasaan Robinson
This study examined whether Virtual Reality technology would have an impact on deception detection when using direct or indirect questions about honesty. Our goal was to identify if someone who feels like they are with a person in a virtual reality world can better perceive facial and body language cues that, when paired with indirect questions, will enhance deception detection. Research has shown that participants are more aware of their surroundings and recognize slight changes more readily in virtual reality. Additionally, much research (e.g., Hippel, 2015; ten Brinke, 2014; Manstead 1984) indicate that indirect questions (focused on a person's jobs, character, and what actions they would take in a given scenario) are more useful than direct questions (e.g., targeted specifically to the honesty of a person) in determining deception. Analyses of data in the present study identified a reliable enhancement effect of virtual reality on body language cues (e.g., facial emotions, fidgeting, posture) as compared with verbal language cues in indicating honesty and deception.
Gabrielle Nicole Bryson
Redlining, the process of denying government backed housing loans from the 1930s until the 1970s, is still reflected in our society today. The purpose of this study was to investigate whether undergraduate students at a predominantly white institution were aware of the topic of redlining based on characteristics such as gender, race, and school year. The aim of this study was to collect the data from the UnDesign the Redline exhibit here at the University of Dayton and through secondary data I was able to find a conclusion to my research question.
Julia Grace Jenkner
The issue of women having a different experience than men in law enforcement agencies deserves to be carefully looked at by others because it is a matter of equality. The purpose of this capstone assignment is to find out if women in law enforcement are held to a different standard than men in law enforcement. The goal is to see if there is a disparity and if so, see if there are similarities or differences between different women in different departments or ranks within a department. The study would accomplish the purpose of this research by gathering information from different women in law enforcement to see if there are similarities in their experiences in law enforcement. My study would accomplish this by gathering information (interviews), asking open-ended questions of participants, analyzing data (put into categories and themes), and looking for patterns in the data.
Kevin Formato Brian O’Neill George Icart Mark Mahoney and Kieran Kilbride
William Sumpter McIntosh was a veteran of the U.S. Navy before residing in Dayton for 33 years. He served as the local director of the Congress of Racial Equality and acted as an advisor to the Dayton chapter of the Southern Christian Leadership Conference. More than 600 people gathered for a public viewing to honor their civil rights leader, and the State Rep. C.J. McLin told the crowd, “He carried a torch. When Mac died, the torch never touched the ground. It is out there for all of us to grab.” That was what he was able to leave behind in his memory. The University of Dayton offers the W.S. McIntosh Memorial Leadership Award to incoming African American freshmen who reside in Montgomery County.
Studies in Drosophila and other tumor models have revealed cancer promoting signaling interactions and transcriptional addictions in tumors cells. The Hippo pathway effector, Yorkie (Yki) is a key mediator of such interactions and presents an attractive opportunity to study transcriptional dependencies in cancer cells. The RasV12 scrib-/- tumor mosaic model is well-established and shows activation of oncogenic Ras in the background of impaired apical-basal polarity. This model is widely used study molecular mechanisms and signaling events downstream of the oncogenic Ras and Ras-mediated Yorkie (Yki) activation in RasV12, scrib-/- tumor cells. Previously, we have shown that in RasV12, scrib-/- cells Wingless (Wg), Caspases (e.g., the initiator caspase Dronc) and JNK are activated to promote tumorigenesis through their non-apoptotic roles. Amongst these, Wg/Wnt pathway is known to act via canonical and non-canonical pathways during development and cancer, and interact with Yki to promote cancer growth. Genetic epistasis showed that Wg acts upstream of Caspases, JNK and Yki, and downregulation of Wg reduced tumor growth by downregulation of Caspases, JNK and Yki reporters. Our goal is to further understand how the two evolutionarily conserved signaling pathways i.e., Hippo and Wingless crosstalk and interact with each other to regulate tumor growth. To understand this intricate wiring of Wingless-Yorkie during tumor growth and invasion, we will use the RasV12, scrib-/- tumor model in Drosophila imaginal discs. Preliminary data showed that wg transcriptional reporters are upregulated in RasV12, scrib-/- cells, suggesting that increased accumulation of Wg may be due to increased transcription. In other contexts, wg is shown as a transcriptional target of Yki. Therefore, we will test for (a) the effects of Yorkie protein, the main effector molecule of Hippo pathway, on wg transcription and expression of other Wg pathway components by reporter assays, and qRT-PCR- based approaches, and (b) feedback interactions that promote tumorigenesis using genetic epistasis-, and immunohistochemistry-based approaches. Here, we present our progress on the organization of the molecular network involving Wingless and Yorkie.